水中細菌計測のための細菌表面構造転写技術の開発

Detection of Bacteria in Water Based on a Transferring Technique of Bacterial Surface Structure

「飲料水・食」の安心・安全の確保および細菌汚染に起因する食中毒や感染症の防止は食品産業や医療業界において重要課題だが，蛍光染色法や培養法を用いた従来法は検査結果を得るまでに数時間〜数日を要する場合があるという課題があった。本研究では，導電性ポリマーに細菌の表面化学構造を精巧に転写した細菌鋳型膜の開発を行い，誘電泳動法と組み合わせてわずか数分で標的細菌を高選択的に検出する新手法の開発に成功した。この新手法を用いることで高選択的，高感度かつ従来法よりケタ違いの速さの安価な水中細菌検査技術も提供できるため，「水」に関わる産業・医療・農業分野の革新につながる成果と期待される。

The development of methods for securing safety of “drinking water / food” (prevention of food poisoning and infectious diseases caused by bacterial contamination) is one of important issues in the food industry and medical industry, whereas conventional methods using fluorescent labeling method and culturing methods often require several hours to several days in order to obtain the inspection results. In this study, we developed a bacterial imprinted film by elaborately transcribing the surface chemical structure of bacteria into a conductive polymer, and successfully pioneers a novel method to selectively detect target bacteria within only a few minutes in conjunction with dielectrophoresis. This new method will provide a simple underwater bacterial inspection technology with high sensitivity, high selectivity and with incommensurable high speed in comparison with conventional methods, leading to the innovation of the industrial, medical, agriculture fields related to “water”.

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