2003年

2003

Solid-liquid extraction room temperature phosphorimetry and pattern recognition for screening polycyclic aromatic hydrocarbons and polychlorinated biphenyls in water samples.

Arruda, A. F.; Goicoechea, H. C.; Santos, M.; Campiglia, A. D.*; Olivieri, A. C.

Environmental Science and Technology, VOL. 37, NO. 7, 2003-04-01, PP. 1385-1391

Both classes of compound were extracted with the same membrane using a syringe kit, and their phosphorescence spectra recorded directly from the surface of a solid support at 90 degree after 5 min of purging with N 2 . measurements were performed with use of a Fluorolog-3 spectrofluorimeter (ISA, Jobin-Yvon-Spex, model FL3-11). The method takes less 8 min and the detection limit for 10 ml of water was at the ppb level. Pattern recognition was used to positively identify PAH, PCB, or both. The method may be suitable for avoiding unnecessary chromatographic analysis of uncontaminated samples. The recognition ability was 100%, while the prediction ability wa close to perfect.

ANALYTE INDEX- 83-32-9 --acenaphthene; 208-96-8 --acenaphthylene; 120-12-7 --anthracene; 56-55-3 --benz" a "anthracene; 50-32-8 --benzo" a "pyrene; 191-24-2 --benzo" ghi "perylene; 207-08-9 --benzo" k "fluoranthene; 92-52-4 --biphenyl; biphenyl derivatives, chloro- --detection of, in water, by phosphorimetry, extraction in; 35694-08-7 --biphenyl, 2,2',3,3',4,4',5,5'-octachloro-; 41464-39-5 --biphenyl, 2,2',3,5'-tetrachloro-; 73575-56-1 --biphenyl, 2,2',3,5,6-pentachloro-; 56558-16-8 --biphenyl, 2,2',4,6,6'-pentachloro-; 37680-65-2 --biphenyl, 2,2',5-trichloro-; 52663-72-6 --biphenyl, 2,3',4,4',5,5'-hexachloro-; 69782-90-7 --biphenyl, 2,3,3',4,4',5'-hexachloro-; 32598-14-4 --biphenyl, 2,3,3',4,4'-pentachloro-; 74472-37-0 --biphenyl, 2,3,4,4',5-pentachloro-; 32774-16-6 --biphenyl, 3,3',4,4',5,5'-hexachloro-; 32598-13-3 --biphenyl, 3,3',4,4'-tetrachloro-; 33284-52-5 --biphenyl, 3,3',5,5'-tetrachloro-; 2050-67-1 --biphenyl, 3,3'-dichloro-; 2050-68-2 --biphenyl, 4,4'-dichloro-; 2051-62-9 --biphenyl, 4-chloro-; 218-01-9 --chrysene; 53-70-3 --dibenz" a , h "anthracene; 262-12-4 --dibenzo- p -dioxin; 3268-87-9 --dibenzo- p -dioxin, 1,2,3,4,6,7,8,9-octachloro-; 58200-70-7 --dibenzo- p -dioxin, 1,2,3,4,6,7,9-heptachloro-; 39227-28-6 --dibenzo- p -dioxin, 1,2,3,4,7,8-hexachloro-; 30746-58-8 --dibenzo- p -dioxin, 1,2,3,4-tetrachloro-; 64461-98-9 --dibenzo- p -dioxin, 1,2,3,6,7,9-hexachloro-; 38178-38-0 --dibenzo- p -dioxin, 1,6-dichloro-; 82306-65-8 --dibenzo- p -dioxin, 1,7,8-trichloro-; 39227-54-8 --dibenzo- p -dioxin, 2-chloro-; 132-64-9 --dibenzofuran; 70648-26-9 --dibenzofuran, 1,2,3,4,7,8-hexachloro-; 24478-72-6 --dibenzofuran, 1,2,3,4-tetrachloro-; 71998-72-6 --dibenzofuran, 1,3,6,8-tetrachloro-; 51207-31-9 --dibenzofuran, 2,3,7,8-tetrachloro-; 5409-83-6 --dibenzofuran, 2,8-dichloro-; 43047-99-0 --dibenzofuran, 2-chloro-; 39001-02-0 --dibenzofuran, octachloro-; 206-44-0 --fluoranthene; 86-73-7 --fluorene; hydrocarbons, polycyclic aromatic --detection of, in water, by phosphorimetry, extraction in; 193-39-5 --indeno"1,2,3- cd "pyrene; 91-20-3 --naphthalene; 85-01-8 --phenanthrene

2003

Self-assembled nanoscale biosensors based on quantum dot FRET donors

IGOR L.MEDINTZ,AARON R. CLAPP,HEDI MATTOUSSI,ELLEN R. GOLDMAN,BRENT FISHER AND J.MATTHEW MAURO

nature materials, SEPTEMBER2003, VOL2, pp.630-638.

2003

Long-term multiple color imaging of live cells using quantum dot bioconjugates.

Simon, Sanford M.; Jaiswal, Jyoti K.; Mattoussi, Hedi; Mauro, J. Matthew

Nature Biotechnology, VOL. 21, NO. 1, January 2003, PP. 47-51.

Luminescent quantum dots (QDs)-semiconductor nanocrystals-are a promising alternative to organic dyes for fluorescence-based applications. We have developed procedures for using QDs to label live cells and have demonstrated their use for long-term multicolor imaging of live cells. The two approaches presented are (i) endocytic uptake of QDs and (ii) selective labeling of cell surface proteins with QDs conjugated to antibodies. Live cells labeled using these approaches were used for long-term multicolor imaging. The cells remained stably labeled for over a week as they grew and developed. These approaches should permit the simultaneous study of multiple cells over long periods of time as they proceed through growth and development.

DESCRIPTOR(S)- *Biochemistry and Molecular Biophysics; *Cell Biology; *Methods and Techniques; *AX2 cell line cell line (Myxophyta, Sarcodina) --Dictyostelium discoideum cells; *HeLa cell line (Hominidae) --American Type Culture Collection; *Animals; *Chordates; *Fungi; *Humans; *Invertebrates; *Mammals; *Microorganisms; *Nonvascular Plants; *Plants; *Primates; *Protozoans; *Vertebrates; *anti-Pgp antibody --Dako; *cell surface proteins; *organic dyes; *quantum dot bioconjugates; *quantum dots QDs --endocytic uptake; *quantum dots QDs --luminescent; *semiconductor nanocrystals; *diode array UV-VIS spectrometer HP8453 --laboratory equipment; *diode array UV-VIS spectrometer HP8453 --Hewlett-Packard; *fluorescence-based applications --imaging and microscopy techniques; *fluorescence-based applications --laboratory techniques; *long-term multiple color imaging --imaging and microscopy techniques; *long-term multiple color imaging --laboratory techniques; *selective labeling --laboratory techniques; *Olympus IX70 microscope --laboratory equipment; *Olympus IX70 microscope --Olympus; *ORCA-ER camera --laboratory equipment; *ORCA-ER camera --Hamamatsu Photonics; *SPEX Fluorolog-3 spectrophotometer --laboratory equipment; *100 W xenon lamp source --laboratory equipment; *470/40 nm band-pass excitation filter --laboratory equipment; *525/50 nm filter --laboratory equipment; *525/50 nm filter --Chroma Technologies; *580/30 nm filter --laboratory equipment; *580/30 nm filter --Chroma Technologies; *590 nm LP --laboratory equipment; *590 nm LP --Chroma Technologies; *620/60 nm filter --laboratory equipment; *620/60 nm filter --Chroma Technologies; *75W xenon lamp --laboratory equipment; *development; *growth; *live cells

2003

Advanced industrial fluorescence metrology used for qualification of high quality optical materials

Halfhill, M.D.; Harkins, D.; Millwood, R.J.; Russotti, R.; Stewart Jr., C.N.

Advanced Characterization Techniques for Optics, Semiconductors, and Nanotechnologies, San Diego, CA, Aug. 3-5, 2003, Bellingham, WA, Society of Photo-Optical Instrumentation Engineers (SPIE Proceedings. Vol. 5188), p. 182-189.

Schott Glas is developing and producing the optical material for various specialized applications in telecommunication, biomedical, optical, and micro lithography technology. The requirements on quality for optical materials are extremely high and still increasing. For example in micro lithography applications the impurities of the material are specified to be in the low ppb range. Usually the impurities in the lower ppb range are determined using analytical methods like LA ICP-MS and Neutron Activation Analysis. On the other hand absorption and laser resistivity of optical material is qualified with optical methods like precision spectral photometers and in-situ transmission measurements having UV lasers. Analytical methods have the drawback that they are time consuming and rather expensive, whereas the sensitivity for the absorption method will not be sufficient to characterize the future needs (coefficient much below 10 exp -3/cm). In order to achieve the current and future quality requirements a Jobin Yvon FLUOROLOG 3.22 fluorescence spectrometer is employed to enable fast and precise qualification and analysis. The main advantage of this setup is the combination of highest sensitivity (more than one order of magnitude higher sensitivity that state of the art UV absorption spectroscopy) and fast measurement and evaluation cycles (several minutes compared to several hours necessary for chemical analysis). An overview is given for spectral characteristics and using specified standards. Moreover correlations to the material qualities are shown. In particular we have investigated the elementary fluorescence and absorption of rare earth element impurities as well as defects induced luminescence originated by impurities.