Who should attend: XelPleX or OpenPlex users People wishing to acquire an XelPleX or an OpenPlex For this training the attendees should be from the same team
Duration: 4 days (From 9 am to 5:30pm)
Learn how to optimize experimental conditions in function of interactions to analyze
Learn how to spot the biochip with the spotter defined by the user
Master the use of the SPRi system, defined beforehand by the user
Learn how to analyze data
Day 1
Which biochip to choose according to the molecules to immobilize?
How to optimize spotting (concentrations, buffers, reference, ...)?
The important experimental parameters to be tested for SPRi (pH, running buffer, temperature,...)
Advice to be applied during the experiment (duration of kinetics, flow rate, regeneration, ...)
Establishment of an experimental protocol
Day 2
Choose suitable surface chemistry for immobilized ligands
Perform spotting based on the previously defined protocol
Advice about the maintenance of the instrument
Day 3
Carry out the SPRi experiment as described in the protocol
Master the use of the chosen SPRi device (XelPleX or OpenPleX)
