モジュール型蛍光分光光度計 Fluorolog-3について記述のある文献一覧です。

掲載年

タイトル

著者

掲載誌

概要

1997

Three-way principal-components analysis for fluorescence spectroscopic classification of algae species.

Henrion, R.; Henrion, G.; Boehme, M.; Behrendt, H.

Fresenius' Journal of Analytical Chemistry, VOL. 357, NO. 5, 1997-03, PP. 522-526

Three-way principal components analysis is presented as the appropriate generalization of conventional principle components analysis as a method of classification of algae species. The investigation was limited to a database consisting of the excitation-emission matrices (EEM) of five species in order to intimate the methodology and potentials of the described approach. The species were selected to represent the main phytoplankton groups. Fluorescence was measured with a SPEX Fluorolog 2 instrument with a 150 W xenon arc lamp and single-grating Czerny-Turner-Monochromators with excitation and emission focal lengths of 0. 227 and 0. 34 m, respectively; slits were fixed at 1 or 2. 5 mm. A PMT Hamamatsu R928 detector was used. Each EEM consisted of 10 excitation spectra from 350-630 nm, step 1 nm; emission was from 660-705 nm, step 5 nm.

1997

The effects of 1-aminocyclopropane-carboxylic acid and inositol-1,4,5-trisphosphate on cytoplasmic calcium concentration during Chinese rose petal development.

Song ChunPeng; Cao GengSheng; An GuoYong; Ma XiaoDong; Du ZuLiang; Zhu ZiQiang; Yan LongFei

Acta Phytophysiologica Sinica, VOL. 23, NO. 1, 1997, PP. 34-42

Protoplasts from Rosa chinensis cv. Zirong petals at different developmental stages were loaded with fura-2-acetoxymethyl ester to measure free Ca2+ concentration in the cytosol. The fluorescence of fura-2 was measured at 450 nm using a Spex F212 Fluorolog with excitation at either 340 or 370 nm. Fluorescence ratio photometry was used to check the method. Ca2+ concentrations at the bud, flowering, wilting and senescing stages were 8, 62, 12 and 15 nmol/litre, respectively. When cut flowers were treated with 1 mmol ACC/litre, petal cytosolic Ca2+ concentration rapidly increased to 108 nmol/litre in conjunction with the premature opening of flowers; no change occurred in the value of Ca2+ concentration during senescence. When the protoplasts were treated with 3.6 micro mol inositol-3,4,5-trisphosphate/litre (IP3), Ca2+ concentration decreased, especially at the opening stage, but this Ca2+ release from protoplasts could be inhibited by introducing La3+, a potent Ca2+-channel blocker, into the cell. It was suggested that the opening of flowers could be induced by a rise in Ca2+ concentration from a relatively constant resting level of 10 nmol/litre and that IP3 affects Ca2+-channel activity in the plasma membrane.

DESCRIPTOR(S)- protoplasts; fluorescence; growth stages; corolla; plant composition; metabolic inhibitors; plant development; cytosol; senescence; flowering; plasma membranes; flowers; development; physiology; calcium; metabolism; cut flowers; plant growth regulators; ACC; responses; ornamental plants; ornamental woody plants
SECONDARY DESCRIPTOR(S)- Rosa; Rosaceae; Rosales; dicotyledons; angiosperms; Spermatophyta; plants
CAS REGISTRY/EC NUMBER(S)- 7440-70-2; 22059-21-8

1997

The effects of 1-aminocyclopropane-carboxylic acid and inosito1-1, 4, 5-trisphosphate on cytoplasmic calcium concentration during Chinese rose petal development.

No Authors;

Acta Phytophysiologica Sinica, Volume 23, Number 1, (1997), pp. 34 -42.

 

1997

Direct Measurement of Molecular - Weight Driven Competition during Polymer Adsorption.

Santore, M. M. and Fu, Z.;

Macromotecules 30, (1997), pp. 8516.