2003年

蛍光分光光度計 SPEX FluoroMaxについて記述のある文献一覧です。

掲載年	タイトル	著者	掲載誌	概要
2003	Measure of meat tenderness using front-face fluorescence spectroscopy.	Frencia, J. P.; Thomas, E.; Dufour, E.	Sciences des Aliments, VOL. 23, NO. 1, 2003, PP. 142-145	This study was carried out in 1999 by ADIV in collaboration with ENITA Clermont-Ferrand. A laptop spectrofluorimeter was developed by ADIV because the ENITA Fluoromax spectrofluorimeter is a bulky machine. The spectroscopic data obtained in this study were compared with sensorial and instrumental texture measurements. To evaluate the spectroscopic method, the spectral data were analysed by multidimensional statistical methods (principal component and canonical correlation analyses). Results showed that fluorescence spectroscopy is a reliable method for the determination of meat tenderness. It was possible to discriminate muscles as a function of the 2 components of tenderness: (1) basic hardness related to the different collagen content varying with the type of muscle and (2) myofibril component related to the proteolysis during meat ageing. In addition, the method is rapid, non-destructive, easy to use, and may be used on-line or at-line in slaughterhouses. DESCRIPTOR(S)- aging; analytical methods; canonical correlation; collagen; correlation analysis; meat; muscles; myofibrils; principal component analysis; proteolysis; sensory evaluation; spectroscopy; statistical analysis; tenderness; texture
2003	Instrumentation and methodology for quantifying GFP fluorescence in intact plant organs	Halfhill, M.D.; Harkins, D.; Millwood, R.J.; Russotti, R.; Stewart Jr., C.N.	BioTechniques, VOL. 34, NO. 3, 2003/Mar 1 2003, PP. p 638-643	The General Fluorescence Plant Meter (GFP-Meter) is a portable spectrofluorometer that utilizes a fiber-optic cable and a leaf clip to gather spectrofluorescence data. In contrast to traditional analytical systems, this instrument allows for the rapid detection and fluorescence measurement of proteins under field conditions with no damage to plant tissue. Here we discuss the methodology of gathering and standardizing spectrofluorescence data from tobacco and canola plants expressing GFP. Furthermore, we demonstrate the accuracy and effectiveness of the GFP-Meter. We first compared GFP fluorescence measurements taken by the GFP-Meter to those taken by a standard laboratory-based spectrofluorometer, the FluoroMax registered trademark -2. Spectrofluorescence measurements were taken from the same location on intact leaves. When these measurements were tested by simple linear regression analysis, we found that there was a positive functional relationship between instruments. Finally, to exhibit that the GFP-Meter recorded accurate measurements over a span of time, we completed a time-course analysis of GFP fluorescence measurements. We found that only initial measurements were accurate, however, subsequent measurements could be used for qualitative purposes. 13 Refs. DESCRIPTOR(S)- Fluorescence; Fluorometers; Optical cables; Proteins; Regression analysis; Tissue

掲載年

タイトル

著者

掲載誌

概要

2003

Measure of meat tenderness using front-face fluorescence spectroscopy.

Frencia, J. P.; Thomas, E.; Dufour, E.

Sciences des Aliments, VOL. 23, NO. 1, 2003, PP. 142-145

This study was carried out in 1999 by ADIV in collaboration with ENITA Clermont-Ferrand. A laptop spectrofluorimeter was developed by ADIV because the ENITA Fluoromax spectrofluorimeter is a bulky machine. The spectroscopic data obtained in this study were compared with sensorial and instrumental texture measurements. To evaluate the spectroscopic method, the spectral data were analysed by multidimensional statistical methods (principal component and canonical correlation analyses). Results showed that fluorescence spectroscopy is a reliable method for the determination of meat tenderness. It was possible to discriminate muscles as a function of the 2 components of tenderness: (1) basic hardness related to the different collagen content varying with the type of muscle and (2) myofibril component related to the proteolysis during meat ageing. In addition, the method is rapid, non-destructive, easy to use, and may be used on-line or at-line in slaughterhouses.

DESCRIPTOR(S)- aging; analytical methods; canonical correlation; collagen; correlation analysis; meat; muscles; myofibrils; principal component analysis; proteolysis; sensory evaluation; spectroscopy; statistical analysis; tenderness; texture

2003

Instrumentation and methodology for quantifying GFP fluorescence in intact plant organs

Halfhill, M.D.; Harkins, D.; Millwood, R.J.; Russotti, R.; Stewart Jr., C.N.

BioTechniques, VOL. 34, NO. 3, 2003/Mar 1 2003, PP. p 638-643

The General Fluorescence Plant Meter (GFP-Meter) is a portable spectrofluorometer that utilizes a fiber-optic cable and a leaf clip to gather spectrofluorescence data. In contrast to traditional analytical systems, this instrument allows for the rapid detection and fluorescence measurement of proteins under field conditions with no damage to plant tissue. Here we discuss the methodology of gathering and standardizing spectrofluorescence data from tobacco and canola plants expressing GFP. Furthermore, we demonstrate the accuracy and effectiveness of the GFP-Meter. We first compared GFP fluorescence measurements taken by the GFP-Meter to those taken by a standard laboratory-based spectrofluorometer, the FluoroMax registered trademark -2. Spectrofluorescence measurements were taken from the same location on intact leaves. When these measurements were tested by simple linear regression analysis, we found that there was a positive functional relationship between instruments. Finally, to exhibit that the GFP-Meter recorded accurate measurements over a span of time, we completed a time-course analysis of GFP fluorescence measurements. We found that only initial measurements were accurate, however, subsequent measurements could be used for qualitative purposes. 13 Refs.

DESCRIPTOR(S)- Fluorescence; Fluorometers; Optical cables; Proteins; Regression analysis; Tissue

蛍光分光光度計・蛍光寿命測定装置

2003年